Analytical method development and validation of diloxanide furoate and ornidazole in its combined pharmaceutical dosage form
نویسندگان
چکیده
A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous estimation of Diloxanide Furoate and Ornidazole in Their Combined Dosage Form has been developed. The separation was achieved by LC20 AT C18 (250mm x 4.6 mm x 2.6 μm) column and Buffer (pH 4.5): Acetonitrile (40:60) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 277 nm. Retention time of Ornidazole and Diloxanide Furoate were found to be 4.620 min and 7.633 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Ornidazole 5-15 μg/ml and for Diloxanide Furoate 7.5-22.5 μg/ml. The percentage recoveries obtained for Ornidazole and Diloxanide Furoate were found to be in range of 100.88 ± 0.60 and 100.85± 0.20 respectively. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial combined dosage form.
منابع مشابه
Assay Method Development and Validation for Simultaneous Quantitative Estimation of Diloxanide Furoate and Tinidazole in Tablets by Rp-hplc
Objective: To develop an accurate, precise and linear Reverse-Phase High-Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Diloxanide furoate and Tinidazole in tablets and validate as per ICH guidelines. Methods: The optimized method uses a reverse phase column, Waters Symmetry C18 (250 X 4.6 mm; 5μ), a mobile phase of triethylammonium phosphate buf...
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